ISTerre - Institut des Sciences de la Terre

Projet Hemi-Breaks-T : Métaux lourds et cassures de l'ADN

Laurent CHARLET — 2025-09-05T13:50:31Z

Métaux lourds et cassures de l'ADN sur différents tissus humains et non humains

Durée : 2011 - 2015

Budget : 140 k€ à ISTerre

Porteur du Projet : INSERM (Lyon)

Equipe ISterre impliquée :
– Géochimie 4D

Contact ISterre : Constance Bochot, Laurent Charlet.

Collaborations :
– U836Radiobiology Group for DSB repair and signalling analyses,
– the CEA-DSV-LRO lab for the cytogenetics ;
– the CNRS-ISTerre for the metal speciation analyses ;
– the IRSN-SECRE for the wors on ecosystems

Heavy metals are abundantly used in a number of industrial activities such as mining and metallurgy, fertilizers, paints, plastics stabilizers, cigarettes, batteries and, more recently, in manufacturing of high-tech products such as mobile phones and CD/DVDs.

Metals (e.g. Cr, Se) and corresponding inorganic nanoparticles (e.g. Se°) represent therefore an actual problem of public health throughout two major features : toxicity and cancer risk. Despite of a considerable number of data, the molecular and cellular bases of the toxicity and/or carcinogenesis of metals remain misknown.

Recently, the development of immunofluorescence has allowed to detect individual DNA damage in each cell nucleus. In parallel, new advances have been made in the understanding of the role of DNA double-strand breaks (DSB), whether non-repaired or misrepaired, in the toxicity and carcinogenesis, respectively.

A number of metals like Fe, Cd, Pb, Zn, Al, Ni, Pd, Cr were shown in a previous project to produce few but severe DSBthat may explain toxicity and genomic instability in human endothelium. Thanks to our new data, we are ready to propose a new system unit with a quantitative scale of toxicity and cancerrisks due to any exposure of metal inducing HEMI-DSBs similarly to the radioprotection Sievert system., ATM, a major actor of the DNA damage repair and signalling, goes from the cytoplasm to the nucleus to control the HEMI-DSB repair via end-joining pathway and inhibits the MRE11 nuclease activity to prevent genomic instability. Interestingly, our groups pointed out that the level of active ATM forms in the cytoplasm and in nucleus are specific to the tissue.

Hence, we proposed here a very ambitious project called HEMI-BREAKS-T that aims to investigate : the chromosome and DNA damage repair and signalling dependent from ATM protein together with the metal speciation in two systems contaminated with metals : HUMAN (brain, skin, lung, liver and blood human cells) and ECOSYSTEM (brain,liver, gonad fish cells).

To this aim, 4 partners will be gathered in this project : the INSERM

The effect of different forms of a given metal (Cr(III), Cr(V) and Cr(VI)) are investigated as well as nanoparticles (e.g. Cr2O3 and Se°).

These studies include :
– Metal speciation, by ESR spectroscopy, in cells contaminated by Cr III, V and VI,
– DSB Repair and Signaling,
Other groups within the programme investigate :
– Cytogenetics
– Consequeces to the ecosystems

This project will provide, for the first time, a coherent and original body of datarelevant for a large number of tissues to better understand the molecular and cellular responses to metal response with the most recent concepts and technology in biochemistry.

Accords transatlantiques et environnement

Laurent CHARLET, Philippe CARDIN — 2014-02-04T11:01:56Z

Des négociations secrètes ont lieu entre les USA et la communauté européenne à propos du Trans-Atlantic Trade and Investment Partnership (TTIP), qui devrait faire suite aux accords NAFTA et TPP. Comme Philippe Baveye et Laurent Charlet (ISTerre) le démontrent dans cet article à travers quelques exemples, toute compagnie pourra alors se retourner contre un gouvernement, tant local que national, si elle considère qu'une "préférence collective", à savoir une loi ou un décret - par exemple touchant à l'environnement, vient entraver ses profits actuels ou potentiels ! Les “Investor-State Dispute Resolution” (ISDR) seront alors réglés par des tribunaux constitués de 3 personnes, hors du système judiciaire !

Proposed Trade Agreements Would Make Policy Implications of Environmental Research Entirely Irrelevant, Philippe C. Baveye and Laurent Charlet

Accords transatlantiques et environnement

Laurent CHARLET, Philippe CARDIN — 2014-02-04T11:01:56Z

Proposed Trade Agreements Would Make Policy Implications of Environmental Research Entirely Irrelevant, Philippe C. Baveye and Laurent Charlet

Assistant Professor Labex Serenade

Laurent CHARLET — 2013-07-16T08:01:27Z

University of Grenoble-I - Assistant Professor in Nanoparticle reactivity, environmental toxicology and ecodesign.

About the job

The University Joseph Fourier (Grenoble, France) seeks a highly qualified candidate for a non tenure-track 3 years long Research Assistant Professor position. The Research Fellow will devote 2/3 of his/her time to research, and 1/3 to teaching (teaching duties : 60 h/year).

Position offer : Research Chair in Nanoparticle reactivity, environmental toxicology and ecodesign

Research Framework : SERENADE, a LABEX (Laboratory of Excellence) network which gathers 15 academic and business partners who work together on the issue of ‘Safe(r) Ecodesign Research and Education applied to NAnomaterial Development' (http://www.labex-serenade.org/)

Salary : Assistant Professor, commensurate with experience and qualification

Teaching

Teaching responsibilities are oriented towards nanotoxicology (toxic mechanisms at the cellular level, target organs, technical challenges regarding the toxicity and genotoxicity tests, procedures and sources of exposure, potential impacts health, regulations), surface chemistry and life cycle of nanomaterials (generalities on nanoparticles, characterization, regulation). In the first years, teaching will be done both within Erasmus Mundus Biohealth Computing Master Program (environmental and animal health track), Biotechnology Master and within the Earth Sciences Groundwater Master :
– 20h (Lectures/TD) UE nanotox M2 BIDT "UE without number as being created now”
– 4h (Lectures) UE Application of biotechnology for Health. M2 BIDT (UE NPHA531B)
– 30h TP biochips. M2 BIDT UE Biochips for health research : practical session (UE NPHA530A)
– 10h (Lectures/TD) M2 Hydrogeology

The long term overall goal is to integrate and translate the work of the other Labex partners into new academic and professional training course, including Nanosafety MOOCS like e-learning. The ability to conduct courses in French is not required but a working knowledge of French language is welcome.

Research :
The research work will be part of the SERENADE Labex project, and will be done in
collaboration with the industrial and academic partners. It will be conducted within one or two Laboratory of the University of Grenoble campus. The candidate is expected to establish a strong research program in toxicology and ecodesign of new nanomaterial-based products, consisting of a multi-scale approach ranging from molecular mechanisms to macroscopic processes associated with, for example, bioreactions, nanotoxicity, genotoxicity, synchrotron based μXAFS, μ tomography or μ XRF. The envisioned research program should also complement and integrate with our current research strengths in Biology and Earth Sciences which include toxicology (see http://biohealth-computing.org), nanoparticle reactivity and biogeochemical fate, and make use of the IAB Proteomics Plateform.

Application File

Qualified candidates should submit an application file that includes a cover letter, CV, a statement of research and teaching interests, contact information for 3 references, and up to three research articles to :

Université Joseph Fourier
DIREVA – Concours SERENADE
Bâtiment administratif
38041 Grenoble Cedex 9

The university position has been officially opened in June 2013, and candidacies will be received until the end of July 2013, A University committee will select the short list in early September, and interview qualified candidates in late September 2013, with a starting date in October or November 2013.

Contacts :

Laurent CHARLET,
Professor, UJF responsible for the LABEX SERENADE project,
ISTerre, University of Grenoble
Tel : + 33 4 76 63 51 98
charlet38 gmail.com
Laurent Charlet is in charge of task PRA 3-1 and co-responsible for task PEI 1
(development of MOOCS like nanosafety e-learning) of the SERENADE Labex

And

Michel Seve
Professor, IAB-UJF
Tel : + 33 4 76 76 54 84
Mseve chu-grenoble.fr
Michel Seve is Head of the Erasmus Mundus Biohealth Computing Master Program
and Proteomics Plateform at IAB-Grenoble

Assistant Professor Labex Serenade

Anaïs SCHNEIDER, Laurent CHARLET — 2013-07-16T08:01:27Z

University of Grenoble-I - Assistant Professor in Nanoparticle reactivity, environmental toxicology and ecodesign.

About the job

Position offer : Research Chair in Nanoparticle reactivity, environmental toxicology and ecodesign

Salary : Assistant Professor, commensurate with experience and qualification

Teaching

Application File

Université Joseph Fourier
DIREVA – Concours SERENADE
Bâtiment administratif
38041 Grenoble Cedex 9

Contacts :

And

Michel Seve
Professor, IAB-UJF
Tel : + 33 4 76 76 54 84
Mseve chu-grenoble.fr
Michel Seve is Head of the Erasmus Mundus Biohealth Computing Master Program
and Proteomics Plateform at IAB-Grenoble

Labex SERENADE : Design des nanomatériaux et Sureté

Géraldine SARRET, Laurent CHARLET — 2012-12-12T15:20:23Z

Safe Ecodesign and sustainable Research and Education applied to NAnomaterial DEvelopment

Durée : 2012 - 2020

Financement : 11 M€ (Agence Nationale de la Recherche)

Coordinateur : CEREGE (CNRS & Aix Marseille Univ.)

Équipe ISTerre impliquée :
– Géochimie

Contacts ISTerre : Laurent Charlet, Géraldine Sarret

Partenaires : LCE, IMBE, LEMIRE, CEA (Cadarache, Marcoule, Grenoble), INSERM & Univ Paris Est, INRA (Nantes, Toulouse, Montpellier), INERIS, Ecole de management Novancia, CCI Paris et partenaires industriels (ARKEMA, ALLIOS)

Le but du labex SERENADE est de créer un réseau dynamique entre laboratories de recherche et industriels sur le design des nanomatériaux de demain, afin d'assurer leur sureté par rapport à l'homme et à l'environnement. Il comprend deux volets, l'un sur la recherche et le second sur la formation.

Les principaux objectifs du programme de recherche sont les suivants :
– développer des produits contenant des nanomatériaux ou des matériaux nanostructurés prenant mieux en compte les risques (exposition et danger) tout au long du cycle de vie,
– sélectionner les tests permettant à une société de conceptualiser le design en optimisant les paramètres nécessaires à la mise sur le marché,
– développer des procédés innovants concernant la fin de vie : recyclage, traitement des effluents et des eaux de surface.

Le projet réunit des compétences et des infrastructures d'expérimentation uniques, indispensables aux acteurs français du domaine pour mener à bien des projets de recherche, développement et innovation et se positionner à l'échelle mondiale sur la thématique des nano-matériaux (EquipexNanoID, iCeintmésocosmes, Ibisa, Animex, NanoBench et Rovaltain).

Le volet éducation a pour but de proposer une formation adaptée pour maitriser les procédés et les nouveaux outils développés dans la thématique des nanomatériaux, sous la forme de cursus universitaires, écoles thématiques, programmes d'échanges, etc..

En savoir plus

Labex SERENADE : Safe nanomaterial ecodesign

Anaïs SCHNEIDER, Géraldine SARRET, Laurent CHARLET — 2012-12-12T15:20:23Z

Safe Ecodesign and sustainable Research and Education applied to NAnomaterial DEvelopment

Duration : 2012 - 2020
Funding : 11 M€ (National Research Agency)
Coordinator : CEREGE (CNRS & Aix Marseille Univ.)
ISTerre team involved : Geochemistry
Partners : LCE, IMBE, LEMIRE, CEA (Cadarache, Marcoule, Grenoble), INSERM & Univ Paris Est, INRA (Nantes, Toulouse, Montpellier), INERIS, Management school Novancia, CCI Paris and industrial partners (ARKEMA, ALLIOS)
Contacts at ISTerre : Laurent Charlet, Géraldine Sarret

The aim of the SERENADE lab is to create a dynamic network between research and industrial laboratories on the design of tomorrow's nanomaterials, in order to ensure their safety in relation to man and the environment. It has two components, one on research and the other on training.

The main objectives of the research program are as follows :

develop products containing nanomaterials or nanostructured materials that take better account of risks (exposure and hazard) throughout the life cycle,
select the tests that allow a company to conceptualize the design by optimizing the parameters necessary to bring it to market,
develop innovative end-of-life processes : recycling, effluent and surface water treatment.

The project brings together unique skills and experimentation infrastructures, essential for French actors in the field to carry out research, development and innovation projects and to position themselves on a global scale on the theme of nanomaterials (EquipexNanoID, iCeintmésocosmes, Ibisa, Animex, NanoBench and Rovaltain).

The education component aims to provide adapted training to master the processes and new tools developed in the field of nanomaterials, in the form of university curricula, thematic schools, exchange programs, etc.

www.labex-serenade.fr

Equipex NanoID : Plateforme d'identification de nanomatériaux

Géraldine SARRET, Laurent CHARLET — 2011-12-12T15:19:00Z

Nanoparticle identification platform dedicated to safety issues

Durée : 2011 - 2019

Financement : 10,2 M€ (Agence Nationale de la Recherche)

Coordinateur : LCSN/LITEN (CEA Grenoble)

Équipe ISTerre impliquée :
– Géochimie

Contacts ISTerre : Laurent Charlet, Géraldine Sarret

Partenaires :
– CEREGE & LCP (CNRS-Aix-Marseille Université),
– U 959 (INSERM),
– LSA-CIME (ANSES)

Site web nanoID

L'equipexNanoID est une plateforme nationale d'identification chimique et de localisation spatiale des nanomatériaux et nanoparticules dans les systèmes complexes à l'état solide, liquide et gazeux (sols, sédiments, milieux aquatiques, organismes, organes, cellules, organites) pour la toxicologie et l'écotoxicologie, afin de répondre aux questions actuelles sur le risque associé à la présence de ces nanomatériaux et nanoparticules manufacturées dans le milieu naturel et dans les organismes vivants.

Les équipements sont constitués principalement :

1. Au LITEN : d'un Microscope Electronique à Transmission couplé à la microanalyse, équipé d'un mode cryogénique, d'un outil de séparation FFF couplé à une ICP-MS (LITEN)

2. Au LCP : d'un spectromètre de masse pour les aérosols (Api-TOFMS)

3. Au CEREGE : des imageurs X en 3D avec des résolutions de 50 nm et 2µm

4. A ISTerre : d'un nanozetasizer pour mesurer la taille et le potentiel zeta des particules en milieu liquide.

Images TEM de nanoparticules d'argent en fond clair et fond noir, qui permet de visualiser les nanoparticules individuelles.

Equipex NanoID : Nanoparticle identification platform

Anaïs SCHNEIDER, Géraldine SARRET, Laurent CHARLET — 2011-12-12T15:19:00Z

Nanoparticle identification platform dedicated to safety issues

Duration : 2011 - 2019
Funding : 10,2 M€ (National Research Agency)
Coordinator : LCSN/LITEN (CEA Grenoble)
ISTerre team involved : Geochemistry
Contacts at ISTerre : Laurent Charlet, Géraldine Sarret
Partners :

CEREGE & LCP (CNRS-Aix-Marseille Université),
U 959 (INSERM),
LSA-CIME (ANSES)

The equipexNanoID is a national platform for the chemical identification and spatial location of nanomaterials and nanoparticles in complex solid, liquid and gaseous systems (soils, sediments, aquatic environments, organisms, organs, cells, organelles) for toxicology and ecotoxicology, in order to answer current questions on the risk associated with the presence of these nanomaterials and nanoparticles manufactured in the natural environment and in living organisms.

The equipment consists mainly of :

1. At LITEN : from a Transmission Electron Microscope coupled to microanalysis, equipped with a cryogenic mode, an FFF separation tool coupled to an ICP-MS (LITEN)

2. At LCP : a mass spectrometer for aerosols (Api-TOFMS)

3. At CEREGE : 3D X-ray imagers with resolutions of 50 nm and 2µm

4. A ISTerre : from a nanozetasizer to measure the size and zeta potential of particles in a liquid medium.

Images TEM de nanoparticules d'argent en fond clair et fond noir, qui permet de visualiser les nanoparticules individuelles.

Hemi-Breaks-T project : Heavy metals and DNA breaks

Anaïs SCHNEIDER, Laurent CHARLET — 2011-01-09T08:55:00Z

Heavy metals and DNA breaks on different human and non-human tissues

Duration : 2011 - 2015
Budget : 140 k€ at ISTerre
Coordinator : INSERM (Lyon)
ISterre Research team involved : Geochemistry
ISTerre contacts : Constance Bochot, Laurent Charlet.
Collaborations :

U836 Radiobiology Group for DSB repair and signalling analyses,
the CEA-DSV-LRO lab for the cytogenetics ;
the CNRS-ISTerre for the metal speciation analyses ;
the IRSN-SECRE for the wors on ecosystems

To this aim, 4 partners will be gathered in this project : the INSERM

The effect of different forms of a given metal (Cr(III), Cr(V) and Cr(VI)) are investigated as well as nanoparticles (e.g. Cr2O3 and Se°).